Journal: Heliyon
Article Title: An in vivo and in vitro assessment of the anti-breast cancer activity of crude extract and fractions from Prunella vulgaris L.
doi: 10.1016/j.heliyon.2022.e11183
Figure Lengend Snippet: (A) The effects of drugs on tumour cell apoptosis. Green spots represent apoptotic bodies, and blue spots represent cell nuclei (400×). (B) The effects of drugs on the expression of cleaved caspase-3 in tumour tissue (400×). (C) Cleaved caspase 3 and corresponding frequencies of TUNEL positive cells expressed as AI. Different marks within treatments indicate significant differences at ∗ p < 0.05 and ∗∗ p < 0.01 compared to the TC group (n = 3). (D) Weight change trends in 10 acutely toxic experimental mice.
Article Snippet: Tumour tissue sections were stained with polyclonal rabbit antibody for pro-apoptotic cleaved-caspase 3 (Affinity Biosciences Cat# AF7022, RRID: AB_2835326 ), according to the manufacturer's instructions.
Techniques: Expressing, TUNEL Assay